mhqD [2019-06-19 10:58:17]

may be involved in protection against methyl-hydroquinone

locus: BSU19560

pI: 5.62

mw: 22.29 kDa

protein length: 200 aa

gene length: 603 bp

function: may be involved in protection against methyl-hydroquinone

product: unknown

essential: no

synonyms: yodD

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,128,464 2,129,066

The protein

Protein family

• [SW|AB hydrolase superfamily] (according to UniProt)

Structure

• [PDB|2H1I] (from B. cereus, 59% identity)

Expression and Regulation

Operons

• genes: [gene|A1CB3DCF2EC78B9B0585CA6AC6F5BB37B8AF0208|mhqE]-[gene|43CD556065D2EF14365C78E014B7B2051CA0C78F|mhqD]
  description: [Pubmed|17725564]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725564], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR]: repression, [Pubmed|17725564], in [regulon|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR regulon]

  regulation

  • induced by 2-methylhydroquinone ([protein|search|MhqR]) [Pubmed|17725564]

Biological materials

Mutant

• MGNA-B435 (yodD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1434 NBRP B. subtilis, Japan]
• BKE19560 ([gene|43CD556065D2EF14365C78E014B7B2051CA0C78F|mhqD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGCTTCATTATAAATTCCC, downstream forward: _UP4_TAAAAAAACCTTAGTCCGGA
• BKK19560 ([gene|43CD556065D2EF14365C78E014B7B2051CA0C78F|mhqD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGCTTCATTATAAATTCCC, downstream forward: _UP4_TAAAAAAACCTTAGTCCGGA

References

• 17407181,17725564